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KMID : 0363219930310010072
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Korean Journal of Dermatology
1993 Volume.31 No. 1 p.72 ~ p.75
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Immunohistochemical Observation of EGF, TGF alpha, and EGF-R in Psoriatiic Epidermis
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하상근
이승철/원영호/전인기/김영표
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Abstract
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Epidermal growth factor(EGF) and transforming growth factor alpha(TGF-a) are polypeptides of 53 and 50 amino acid residues.
Both bind to epidermal growth factor receptor (EGFR-R) leading to phosphorylation of the receptor, enhancement of its tyrosine-specific kinase activity and ultimately to stimulation of cell growth.
To study he role of EGF, TGF-a, and EGF-R in differentiation and hyperproliferation of cell, we selected psoriasis vulgaris, because the affected keratinocyte mayhave both an abnormally increased proliferative capacity and an abnormally
differentiated
state.
The biopsy specimens were taken from involved and uninvolved skin of 20 patients with psoriasis and immunoperoxidase studies with formalin-fixed paraffin-embedded tissues were perfored with EGF, TGF-a and EGF-R using the Vectastain ABC
immunoperoxidase
stain system. The antibodies were used at a concentration of 6μg/ml
In involved psoriatic skin, distributions of TGF-a and EGF-R were increased in all layers of epidermis as compared to normal, uninvolved psoriatic skin, in which they were showed to the basal and parabasal layers. However, distribution of EGF was
weekly
positive in the basal layers of epidermis in both involved and uninvolved skin with no difference between them. These results suggest that increased distribution of TGF-a and EGF-R may be involved in hyperproliferative state of epidermal
keratinocytes
in psoriatic lesion. (Kor J Dermatol 1993 ; 31 (1): 72-75)
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KEYWORD
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